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Finally, platelet-free plasma was ultracentrifuged at 100,000 × g, 4 °C for 45 min to pellet submicron vesicles.

A small portion of intact MPs from each rat was used to determine the protein concentration prior to in vitro treatments.

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During late-stage (8-weeks) PAH, but not early-stage (3-weeks), an increase in ICAM-1 was observed.

We investigated the presence of intercellular adhesion molecule-1 (ICAM-1) in both PAECs and PMVECs to determine if the two cell types had unique responses to circulating PAH MPs.

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